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SUMMARY:UNIT 6
DTSTART;TZID=America/Los_Angeles:20260501T190000
DTEND;TZID=America/Los_Angeles:20260501T200000
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DESCRIPTION: Sanger sequencing is the process of incorporation of chain-terminating dideoxynucleotides by an enzyme called DNA polymerase. Dideoxynucleotides (ddNTP) prevents DNA strand extension/ further DNA polymerization. This is due to dideoxy not having an OH at the 3' end for the next nucleotide to bind to. In the Sanger sequencing process a mixture of normal deoxyribonucleotides and ddNTP is added. The ddNTP can be incorporated at any point in the growing chain, and when it does, DNA terminates at that point. Because of its random nature, addition of a particular ddNTP will result in a set of different lengths of DNA terminating at the aforementioned nucleotide. 4 different ddNTPS are used in 4 separate DNA synthesis reactions, each reaction produces a set of DNA copies that terminate at different points in the sequence as seen in Figure 1 below.

Figure 1. Sanger Sequencing method.
The growing DNA chain is formed with the help of a template strand. The complementary nucleotide of each...

https://www.learndesk.us/class/6031358776311808/lesson/5092158f63b00030e357b57e019d52b2?ref=outlook-calendar
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