Now let us study the transmission electron microscope or TM for short. Here is a brief outline for tm, we will see what is it used for how it works the electron pathway, a tm machine an example of tm images. p m is used to visualize structures in ultra thin sections of cells. Shown here are the set of models bacterial cells in cross sections that are ultra thin. This is how the works first the specimen is mounted onto a suitable plastic, medium or frozen Next, ultra thin sections of the prepared specimen must be cut. This is important because the electron beam can only penetrate a very short distance.

For this purpose a diamond knife can be used to cut the specimens into thin sections. A single bacterial cell can be sliced into hundreds of sections that's similar to slicing a loaf of bread. Then several sections are placed on a small grid and stain with heavy metals such as lead and osmium. The grid is inserted into the vacuum changer chamber. Next, the machine focuses 100,000 volt electron beam on portion of the section at a time forming an image. The image can be magnified up to 2 million times.

Shawn here is the electron beam going through the anode, the condenser lens, objective lens diffraction and projector lenses, after which the electron beam is captured by the viewing screen. The sample being placed at the level of the objective lens. Shown here is the T machine and a schematic of the same showing the lenses the electron source and once the final image is formed Khalid transmitted to the television shilling here are tm images of flagellated salmonella in a non motile Shigella bacteria in V, both of which are the second and third most common causes of foodborne disease in the United States. In seeing you see a tm image of herpes virus found in the within the nucleus of the cell over here, this being the nuclear membrane and indeed, an image of mitochondria within the cell, showing the matrix and Krista