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URL:https://www.learndesk.us/class-it/6027103176753152/lesson/4bb06f9eb05142a703cc7afa83175780?ref=outlook-calendar
SUMMARY:The Magic of Enzymatic Analysis (La magia dell'analisi enzimatica)
DTSTART;TZID=America/Los_Angeles:20260513T190000
DTEND;TZID=America/Los_Angeles:20260513T200000
LOCATION:https://www.learndesk.us/class-it/6027103176753152/lesson/4bb06f9eb05142a703cc7afa83175780?ref=outlook-calendar
DESCRIPTION: The Magic of Enzymatic Analysis
Now that you understand how a spectrophotometer works, let me show you how you can use this for a wide variety of very precise analyses of organic materials that are in wine.
Let's start with malolactic fermentation, which we've already learned how to look at using paper chromatography and I briefly mentioned that it could also be measured with a spectrophotometer.
Just to review that, we can measure malic acid by oxidizing it using NAD-plus, which is reduced to NADH, which absorbs light at A340. That's just at the lower detection limit of a visible spectrophotometer and certainly no problem for one with UV capability. That's the way people buy them these days – what's called UV-vis – that can do both. What it actually does is oxidize it to pyruvic acid, and we measure the change in NADH before and after.
There's a similar analysis for lactic acid, so we can measure that, too. I'd like to do that because if we have a white, we want...

https://www.learndesk.us/class-it/6027103176753152/lesson/4bb06f9eb05142a703cc7afa83175780?ref=outlook-calendar
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